# Histology

**URL:** <https://network.greenyourlab.org/t/histology/286>\
**Category:** Specialty Labs\
**Created:** [May 26, 2021, 1:08am UTC](https://network.greenyourlab.org/t/histology/286 "2021-05-26T01:08:47Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![GreenYourLab](https://yyz2.discourse-cdn.com/free1/user_avatar/network.greenyourlab.org/greenyourlab/32/517_2.png) [@GreenYourLab](https://network.greenyourlab.org/u/GreenYourLab)\
**Post date:** [May 26, 2021, 1:08am UTC](https://network.greenyourlab.org/t/histology/286/1 "2021-05-26T01:08:47Z")

</div>

Branch of scinece that studies microscopic anatomy of biological tissues.

# Staining

Hematoxylin-Eosin is a commonly used tissue stain. Hematoxylin stains nucli blue and eosin stains the extracellular matrix and cytoplasm pink. This techniques uses hazardous Xylene as a deparaffinizing agent. Exposure also occurs during tissue processing, placing the coverslip and cleaning.

Try replacing it with dish washing solution or other non-hazardous alternatives.

[Prema et al.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595545/) showed that all three - 17% dishwashing soap, a 95% lemon water and a 100% coconut oil - could be used as a safer and cost-effective substitute for xylene. All gave good results.

[Sravya et al.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917531/) processed tissue with sesame oil stained with 1.7% dish washing solution as an alternative to xylene.

[Pandey et al.](https://pubmed.ncbi.nlm.nih.gov/25328332/) also reported dishwashing solution as a great alternative to xylene.

[Premalatha et al](https://pubmed.ncbi.nlm.nih.gov/23811660/). used mineral oil as a substute for xylene.

# Heading 1

## Heading 2

### Heading 3

_This article is a stub. You can help by expanding it. Get Involved!_